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KMID : 0380020080230060554
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 6 p.554 ~ p.556
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Expression of recombinant protein from Oenanthe javanica DC. leaf tissues as a biofactory
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Abstract
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Fresh Oenanthe javanica DC. leaves still attached to stem architecture were immersed in NaOH solution for 3 min before agroinfiltration and co-cultivation. MTT assay revealed that NaOH solution containing up to 0.7% was still safe for the leaf viability. Fluorometric GUS enzyme analysis showed that 0.5% NaOH-treated leaf tissues were efficiently transformed by vacuum infiltration for 20 min with Agrobacterium cells at a density of OD600=0.5 to 1.0. These conditions worked well for the expression of HBsAg, which was confirmed by western blotting and ELISA.
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KEYWORD
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Oenanthe javanica DC, leafy tissue, MTT, biofactory, HBsAg
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